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Protein/macromolecular crystallography

3D structure of large molecules for rational drug design

This technique employs single crystal X-ray diffraction (SXRD) to unambiguously determine the three dimensional structure of large biological molecules. Pharmaceutical and biotechnology researchers need to understand how disease is controlled at the molecular level within proteins and related macromolecules so as to target specific entities based on this knowledge. A prerequisite for this approach is the ability to obtain a high-resolution 3D molecular structure of the macromolecule under investigation. Given the importance of this research, Rigaku has been at the forefront in the development of a wide range of instrumentation related to various aspects of macromolecular crystallography.

In medicine, biotechnology and pharmacology, drug discovery is the process by which drugs are discovered and designed. In the recent past, most drugs have been discovered either by identifying the active ingredient from traditional remedies or by serendipitous discovery. A new approach, using so-called "rational drug design", has been to understand how disease and infection are controlled at the molecular and physiological level and to target specific entities based on this knowledge. Given this importance, Rigaku has been at the forefront in the development of single crystal diffraction for both macromolecular and small molecule structure determination. Our products range from automated crystallization and crystal imaging through crystal diffraction screening and high-resolution data collection systems to 3D structure refinement and imaging software.

Protein crystallography

Rigaku recommends the following products:

  • Fast, flexible single crystal X-ray diffractometer with the latest generation sources and HPC X-ray detectors, perfect for any crystallography lab.

  • Single crystal X-ray diffractometer with high-flux microfocus rotating anode X-ray generator

  • Versatile and high-flux dual-wavelength (DW) X-ray diffractometer with HPC X-ray detector for multipurpose diffraction experiments

  • User-inspired data collection and data processing software for small molecule and protein crystallography.

  • Automated tool for performing in situ crystallography experiments on existing X-ray diffractometers

  • Automated crystal transport, orientation and retrieval robot

  • Low noise, sensitive, air-cooled X-ray detector for superior X-ray diffraction data quality

  • PILATUS3 R hybrid pixel array detector (HPAD) for X-ray crystallography

  • Small angle X-ray scattering (SAXS) Kratky camera system

  • Single crystal X-ray diffractometer with custom enclosure and flexibility for easy integration of accessory components

  • High resolution X-ray detectors for X-ray diffractometers from DECTRIS

  • Single wavelength Confocal Max-Flux (CMF) optics for protein crystallography

Application notes

The following application notes are relevant to this technique